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Activation requires ligand binding at both glutamate and glycine sites, while magnesium blockade at resting membrane potential ensures voltage-dependent activity [96]

Later experiments have found that adding high doses of copper can increase reactive oxygen species and protein carbonylation levels, decrease glutathione levels and SOD enzyme activity, leading to depolarization of mitochondrial membrane potential, the release of cytochrome c, elevated levels of active caspase-9 and caspase-3, increased levels of Bcl2-antagonist/killer 1 (Bak1) and Bcl2-associated X protein (Bax), and decreased levels of B-cell lymphoma 2 (Bcl2), ultimately resulting in cell apoptosis (134)

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Involvement of transcription factors in plasma HDL protection against TNF-alpha-induced vascular cell adhesion molecule-1 expression