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The calculated activation energies (G in kcal mol -1 ) for the abstraction the C2 proton of 7 to produce the cyanidin enolate ( 8-enolate) were found to be almost identical for the wild-type and the C13S variant, in agreement with their similar dehydratase activity measured experimentally

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Flow cytometry Cells from in vitro experiments were collected on ice in 10 mM EDTA in PBS and fixed with 4% paraformaldehyde before flow cytometric analysis

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