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Sputum neutrophil elastase associates with microbiota and Pseudomonas aeruginosa in bronchiectasis

The column was eluted isocratically at a flow rate of 450 L/min for 1 minute at 80% mobile-phase A (10 mM NH 4 Ac/methanol/acetic acid 95:5:0.1, by volume), followed by a linear gradient to 80% mobile-phase B (methanol/acetic acid

Subsequently, 0.1 mM IPTG was added to the transfected cells, which was then cultured with 1 L LB medium for 16 h at 27 C

This means running a parallel experiment with a vehicle solution (the bacteriostatic water without the peptide) to ensure the observed effects are genuinely from the AHK-Cu and not some other variable

Peptide hydrolysis accelerates dramatically above 8C