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Membranes were blocked during 1 h at room temperature in 1 PBS containing 0.1% Igepal and 5% milk and then incubated overnight at 4C with the following primary antibodies: anti-CBS mouse monoclonal antibody (ab12476, Abcam, 1:1,000) and anti--tubulin mouse monoclonal antibody (T6793, Sigma-Aldrich, 1:10,000)

15 BeilschmidtL

Pre-security on the second floor of Terminal H, MIA Health offers therapeutic and relaxation massage to relieve pain and improve circulation, along with IV therapy, rehydration and vitamin infusions for energy, immunity and recovery

Spear, L

Cellular experiment Cardiac Muscle Cell Line HL-1 cardiomyocytes (Sigma-Aldrich, USA) were cultured in DMEM and incubated at 37 C in a humidified incubator with 5% CO 2