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Supplementary Note 2) and HA-URA3 as BamHIKpnI fragment into the polylinker of pRS314 serving as templates for pJD646 ( Ub-F-DHFR-wt ), pJD647 ( Ub-F-DHFR-P67L = K1 ) and pJD648 ( Ub-F-DHFR-T39A , E173D=K2 )

Significant degradation can occur rapidly, compromising the compounds efficacy

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