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Generally, 1 L of EA was diluted with 87 L of pH 7.4 buffer (50 mM TrisHCl, 1 mM CaCl 2 , 100 mM NaCl, and 2 mg/mL N,N dimethylcasein) and 5 L dithiothreitol (20 mM) at 37 C followed by the addition of 2 L of human FXIIIa (0.3 M) and incubation for 10 min

The compromised ocular surface is then predisposed to thinning and perforation which are the end stages of Neurotrophic keratitis

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Laboratory and animal models suggest a role in connective tissue repair, although how meaningfully this translates to a healing human knee is still being investigated

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