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qPCR was performed using AceQ Universal SYBR qPCR Master Mix (Vazyme, China) as following reaction conditions: one cycle at 95C for 10 min, 40 cycles of amplification at 95C for 10 s, 58C for 15 s, and finally 72C for 20 s in a QuantStudio 3 (Thermo, USA) system

This phenomenon, known as reperfusion injury, involves the oxidation of cellular components, mitochondrial dysfunction, and the activation of inflammatory pathways [91, 92]

1,4-benzenediol (493 mg, 4.56 mmol) was dissolved in a mixture of water (50 mL) and 60% perchloric acid (3.6 mL)

Consult the healthcare professional and check the regulations before you proceed with the treatment

On the other hand, the 13 and the 49 segments of the M105I system were closer together because of the folding suffered by the mutant system (Supplementary Fig