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Analyses were performed with an Agilent 1290 Infinity Ultra-High Performance Liquid Chromatography (UHPLC) system (Agilent, Santa Clara, CA, USA) coupled with an MS/MS Sciex Triple Quad 6500 (AB Sciex LLC, Framingham, MA, USA) using electrospray ionization (ESI) in positive mode and according to the manufacturers instructions

A significant body of work, often based on neuroimaging techniques such as positron emission tomography (PET) and blood-oxygen-level-dependent functional magnetic resonance imaging (BOLD-fMRI), has reported a disproportionate increase in glucose utilization relative to oxygen consumption during brain activation

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Maternal malnutrition whilst pregnant may cause abnormal neurodevelopment in the fetus, which may result in related problems in the adult offspring