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3T3-L1 pre-adipocytes cells were cultured with standard culture media (DMEM, 4.5 g/L glucose, L-glutamine, sodium pyruvate, 10% FBS, 1% antibiotic-antimycotic solution) and grown for 48 h before initiating differentiation using the manufacturer's suggested protocol and modified from previous published work

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HUBERMAN: And this is actually going to be a pretty quick discussion because really the main one in this category, besides thymus and beta-4, remember thymus and beta-4 we talked about earlier, this is a peptide that's naturally released from the thymus and the thymus is a structure that depletes over time as children age