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For reduction and alkylation, 20 l of each sample corresponding to 40 g of protein was mixed with 20 l of a solution of 40 mM CAA20 mM TCEP and incubated at 37 C for 30 min

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10 FR is unique among the FRs in that it is a secreted protein: it lacks the C-terminal hydrophobic glycophosphatidylinositol (GPI) membrane anchor that is found in all other FRs

In the Valproic acid-induced autism spectrum disorder model of rats, oral administration of 20, 40, and 80 mg/kg of BM methanolic extract for 20 days decreased the inflammation in the hippocampus and prefrontal cortex by decreasing the IL-1, IL-6, and TNF- levels dose-dependently