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Lipids were separated at a flow rate of 0.3 mL/min and a column temperature of 50C by a gradient elution starting at 10% B increasing in 20 min to 80% B, followed by an increase to 95% B within 4 min, and further 3 min until 100% B is reached and kept for 5 min, followed by a decrease to 10%B within 0.1 min and equilibration at 10% B for 7.9 min 56

Appl Microbiol Biotechnol

About 200 worms were picked, washed with 200 mM MES, 50 mM phosphate and 1 mM EDTA, pH=6 buffer and flash frozen in liquid nitrogen

Efficacy and safety of sirolimus in patients with systemic lupus erythematosus: A systematic review and meta-analysis