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Acknowledgements The author express their thanks and gratitude to AlMaarefa University, Riyadh, Saudi Arabia for the support to publish this article

From these data, tricarboxylic acid (TCA) cycle intermediates, amino acids, fatty acids (FAs), and their derivatized, nucleotides etc., were the most significant metabolites distinguished as potential variables which could discriminate healthy subjects from thyroid nodule cases

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Mild allergic reactions (rash, itching, mild swelling) sometimes seen even with proper technique

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