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Description

The amine groups of preferably lysine groups are modified with (sulfo)-SMCC rendering thiol-reactive maleimide groups on the protein

At high doses, a small proportion of B12 is absorbed passively through the gut lining without requiring intrinsic factor, which is sufficient to maintain adequate levels in many patients

Cells were encapsulated into droplets and libraries prepared using the 10x Genomics platform (Hangzhou Cosmos Wisdom Biotech Co., Ltd.), and libraries were sequenced on an Illumina NextSeq