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Colon samples were heated in 10 mM Citrate buffer (pH 6) for 15 min and then were incubated with Proteinase K (Sigma-Aldrich, St

Bac water has 0.9% benzyl alcohol as a preservative and contains no salt

We did not examine naturally mated queens due to the complications of controlling the timing and frequency in which they would partake in mating flights and complete their mating cycles

This leads to the downregulation of tight junction proteins such as ZO-1, occludin, and claudin-1, thereby significantly increasing intestinal permeability (Zhan et al., 2025)

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