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Peptides are fragile molecules that degrade when exposed to heat, light, moisture, and contamination

The supernatants were transferred into a new tube containing a SpinX HPLC 0.2-m nylon microcentrifuge filter (Costar, Corning, NY, United States), centrifuged again at 10,000 g for 2 min at 4C, and stored at 80C until used

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low-altitude Hu lambs were enriched in some common GO terms (e.g., extracellular matrix organization in the kidney, localization within membrane in the cerebrum and cellular response to angiotensin in artery) that could be directly activated by hypoxia 55 (Fig

After bath sonication in a water-ice mixture for 3 min at 47 kHz (with 30 s of manual shaking at every minute), the suspension was centrifuged (11,000 g , 4 C, 10 min) to obtain the virus-containing supernatant