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This lifelong addition creates a characteristic age gradient: the nucleus contains the oldest fibers, while the cortex holds progressively younger ones

In vitro Methyltransferase assays The DNA sequences corresponding to mouse Mettl9 -FLAG and Stmn1 -HA were PCR amplified by a Phusion Plus DNA Polymerase (Thermo Fisher, #F630S) from pyCAG-METTL9-FLAG and pyCAG-STMN1-HA plasmids respectively (pyCAG plasmids generated in this study, as explained in other sections) with PCR primers (Supplementary Data File 4) including EcoRI and XhoI restriction sites

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10.3892/or.2019.7189 Oncol