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To directly validate the proposed roles of Vit A and Lup in sperm cryopreservation, future work could employ western blot analysis to quantify the expression and activation states of key proteins, such as NRF2 and Caspase 3, along with their downstream effectors, in sperm following freezethaw

To investigate the function of MrGST1 in anthocyanin transport, 35S::MrGST1 was transformed into the Arabidopsis mutant tt19 , a knockout mutant of the anthocyanin transporter AtTT19

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