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The essential impact of selenium is largely realized through its incorporation into the foundational family of selenoproteins, such as GPX and TXNRD [59]

[16] Pancreatic exocrine insufficiency can interfere with normal dissociation of vitamin B 12 from its binding proteins in the small intestine, preventing its absorption via the intrinsic factor complex

Also valine has been proven important for the oxidation of the reduced flavin by molecular oxygen in choline oxidase and it has been reported that replacement of Val464 with alanine in the enzyme results in a twofold decrease in the limiting rate constant for flavin reduction (k red ) and less than fivefold decrease in the equilibrium constant for formation of the enzymesubstrate complex (K d ) 52

Ryskalin, L., Limanaqi, F., Frati, A., Busceti, C

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