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We believe this additional context regarding the number of values used to calculate the median, 10th percentile, and 90th percentile allowed amountsbecause more price volatility might reasonably be anticipated with respect to a less frequently performed servicewould help MRF users determine whether allowed amounts are reasonably good approximations of what typically would be generated by the payer-specific negotiated charge percentage or algorithm

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Moreover, OPLS-DA models were validated by internal iterative cross-validation with seven rounds of permutation test response (800 repeats) not clear, and CV-ANOVA (ANOVA testing of cross-validated predictive residuals)