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Protein determinations Cells or tissues were lysed in 0.1 M NaOH and used for the determination of protein concentrations, either by the method of Lowry et al ., as described 36 or by the BCA protein assay kit (Pierce), following the manufacturer's instructions

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(B) Western blot demonstrated a higher level of Nrf2 protein expression in the glutathione ethyl ester (GSH-EE) group and the control group compared to the normal group at different time points, though there was no significant difference between the GSH-EE group and the control group at 2, 6, and 12 hours after the last ovalbumin (OVA) challenge (the bar indicated mean SEM, n=8 for control group and GSH-EE group, n=5 for normal group)