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doi:10.3389/fnins.2015.00335

High-throughput screening assays RNA bind-n-seq assay and analysis For the RNA bind-n-seq assay, a single-strand DNA library containing a randomized 40-nucleotide region was obtained from IDT, gel-purified, and the RNA library was prepared following a previously described method 48 using a T7 promoter and in vitro transcription

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This pathological condition is characterized by an initial restriction of blood flow to an organ, followed by restoration of perfusion and oxygenation, which paradoxically induces further damage through mechanisms such as oxidative stress and inflammation [1]