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doi:10.1016/j.lfs.2006.11.043 Rothfuss A, Speit G (2002) Investigations on the mechanism of hyperbaric oxygen (HBO)-induced adaptive protection against oxidative stress

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The three libraries were generated using error-prone PCR as described above, using the following conditions to produce varying levels of mutagenesis: Library 1: 2 M 8-oxo-dGTP, 0.5 M dPTP, 20 PCR cycles Library 2: 2 M 8-oxo-dGTP, 1 M dPTP, 20 PCR cycles Library 3: 2 M 8-oxo-dGTP, 2 M dPTP, 20 PCR cycles The library sizes (approximated by number of transformants as described above) were 6 10 7 for each library

Unlike conventional glutathione, which is undetectable in the blood after just 10 minutes, our S-Acetyl Glutathione remains active and detectable for 50-60 minutes

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