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We determined cell viability and cytotoxicity by using tetrazolium reduction assay and Lactate Dehydrogenase (LDH) assay, performed real-time PCR and protein quantification to assess the expression of biomarkers after treating cells with copper peptide (GHK-Cu), copper chloride (CuCl 2 ) and copper acetate (Cu(OAc) 2 )

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It serves as the starting amino acid for protein synthesis in nearly every cell and is the primary dietary source of sulphur used in dozens of metabolic reactions

After these promising results, the European Group on Graves Orbitopathy (EUGOGO) performed a randomized, placebo-controlled, multicenter, clinical trial in European countries known to have marginal selenium-deficiency, to investigate the effect of selenium and pentoxifylline in mild GO ( Figure 2 )

Dose Dependence -Alanine is not an approved treatment for AD, and its doseresponse relationship in this particular context has not been established