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A We recommend the following method (see table)

Cytotoxicity assay Upon necrotic cell damage cells lose membrane integrity and cytoplasmic contents can leak out

Reconstitute lyophilized BPC-157 using bacteriostatic water

Collectively, these agents converge on the Nrf2, SIRT1, and AMPK signaling networks to restore redox homeostasis, prevent mitochondrial dysfunction, and inhibit oxidative stressinduced apoptosis, ultimately improving follicular viability and oocyte developmental competence during cryopreservation and transplantation