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Further elucidation of the exact role of O-GlcNAcylation in HD pathology will lead to a better understanding of the molecular etiology and pathophysiology of HD

Denaturing immunoprecipitation Cells were lysed with 100 l of SDS-containing buffer (50 mM Tris pH 7.5, 1% SDS, 2.5 mM NEM) for 10 min on ice, scraped and SDS was diluted with 900 l dilution buffer (50 mM Tris pH 7.5, 150 mM NaCl, 0.5 mM EDTA, 0.5% NP40 and freshly added 5 mM DTT, protease and phosphatase inhibitors)

[DOI] [PMC free article] [PubMed] [Google Scholar] 9.Braillon A., Lang A.E

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Islet-specific CTL cloned from a type 1 diabetes patient cause beta-cell destruction after engraftment into HLA-A2 transgenic NOD/scid/IL2RG null mice