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To measure the ATPase activities of ABCC2 against different bilirubin ditaurate (BDT, disodium salt) (GC42931-10, GLPBIO) or estradiol-17-D-glucuronide (E 2 17G, disodium salt) (GC10964-10, GLPBIO) concentrations or varying ATP concentrations, protein at a final concentration of 0.05 M was added to 75 L reaction buffer containing 50 mM Tris-HCl, pH 7.4, 50 mM KCl, 1 mM DTT, 0.06% (w/v) digitonin, 2 mM MgCl 2

Prx5 has a cytoprotective effect against H 2 O 2 - and lipid hydroperoxide-generated oxidative stress 33

Furthermore, antioxidant defenses are essential for maintaining Treg suppressive capacity, and treatment with N-acetyl cysteine (NAC), an ROS scavenger, can reverse Treg dysfunction induced by elevated ROS levels (84, 85)