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Description

Plasmid construction Hrd1- pDsRed/pCMV-HA/pCMV-FLAG, Hrd1 -C-pCMV-HA, Hrd1 -R-pCMV-HA, Hrd1 -R-RING-pCMV-HA, MafA -pCMV-MYC, MARE-luc, Scd1 -pEGFP/pCMV-MYC, Scd1 -H153R-pEGFP, Scd1 -H153157R-pEGFP, Ubiquitin-pCMV-HA were generated using a QuikChange Site-Directed Mutagenesis kit (Stratagene, La Jolla, CA, USA) as previously described [17]