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Methanogenesis appeared active in the 612 cm sediment layer at site 9.2, where genes such as hdrA2 (135 tpm), mvhA (85 tpm), and cdhE (33 tpm) were strongly upregulated compared to other samples, where they were almost absent ( 50 tpm), downregulated at 612 cm in S9.2_Core6 (11 tpm), and upregulated in the mat-rich sediment of S9.2_Core10 (150 tpm)