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[6] Set that beside the 24 minute half-life and the mismatch is stark: a compound essentially cleared from plasma within 20 minutes, reported to begin acting at 60 minutes and to persist for up to 20 hours

Based on results of SPR, the PV2-PV3 fusion protein demonstrated a kD value of 63.5 nM, while the PV2-S71 + PV3-N77 combination exhibited a significantly enhanced binding affinity with a kD of 6.34 nM

For immunoprecipitation experiments of the 1 ectodomain, we used ProteinG (Sigma) or M2-agarose (Sigma)

Kasuya, F., Hiasa, M., Kawai, Y., Igarashi, K

It is involved in maintaining essential physiological functions