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Glutathione depletion up-regulates Bcl-2 in BSO-resistant cells

For experiments requiring manipulation of cystine availability, cells were cultured in RPMI 1640 medium without L-glutamine, L-cysteine, L-cystine, and L-methionine (Fisher Scientific, ICN1646454) supplemented with 5% dialyzed FBS (Sigma-Aldrich, F0392), L-glutamine (VWR, VWRL0131-0100), and L-methionine (Sigma-Aldrich, M5308)

The inherent pathological changes of stroke have been shown to be closely related to the characteristics of ferroptosis, including iron metabolism disorders, lipid peroxidation, and elevated ROS levels

Studies confirm that nad infusion with glutathione helps boost insulin sensitivity and glucose metabolism