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After another centrifugation step for 15 min at 20,000 g and 4 C, 5x Laemmli buffer containing 10% -mercaptoethanol (-Me) was added to the samples

Kostic M, et al

In addition, nearly one third of the population is missing the genes that allow them to metabolize glutathione properly

Appl Microbiol Biotechnol 96:13831394 Syngai GG, Gopi R, Bharali R, Dey S, Lakshmanan GM, Ahmed G (2016) Probiotics - the versatile functional food ingredients