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Your doctor may order special tests for antibody levels to determine if you have pernicious anemia

Common symptoms of a vitamin B12 deficiency include: Tiredness Lethargy Feeling faint Breathlessness Less common symptoms include: Headaches Heart palpitations Altered taste Loss of appetite Ringing in the ears (tinnitus) Looking pale Sore mouth and tongue If it is left untreated you can end up with longer term problems including: Psychological problems such as depression, confusion, memory problems Nerve problems such as numbness, pins and needles, vision changes and unsteadiness A blood test can indicate if you have a B12 deficiency

The supernatants were discarded and the cell pellets were resuspended in a lysis buffer (1.5 mL per gram of cell pellet)

The water vapor is then condensed back into its liquid form in another container