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Arora, J

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TNF- triggers hepatocyte apoptosis, necrosis, liver inflammation and regeneration, autoimmune hepatitis and HCC, making it a promising diagnostic biomarker and appealing therapeutic target for NAFLD-associated HCC (135) demonstrated that TNF- inducible gene 6 protein and its derived peptide could ameliorate the liver fibrosis in mice with ALD by restraining CD44 activation, so as to be a treatment approach for ALF

Pull-down assay by FLAG-Sirt7 To a 150 L of solution of nucleosomes (0.05 M) and FLAG-Sirt7 (WT, DM, or CA) (0.17 ng/L) in buffer A (20 mM Tris (pH 8.0), 100 mM NaCl, 5 mM MgCl 2 , 1 mM DTT, 2.5% glycerol, 0.01% Triton X-100), 5 L of anti-FLAG M2 magnetic beads (Sigma, #M8823) in 50 L of buffer A was added

Complementary, this finding was confirmed by flow cytometry (flow cytometry plots: Fig