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Inhibiting COX-2 attenuated the rise of COX-2 levels and epithelial-mesenchymal transition (EMT) scores in NPC cells driven by MDSCs, suggesting COX-2 as a critical checkpoint for MDSCtumor cell interactions and tumor promotion [47]

Bevacizumab (25 g/mL) decreased neoangiogenic cord formation by 70%, but only reduced established cord formation by 20% (Figure 4b)

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After incubation of the secondary antibody, Alexa Flour 488-conjugated goat anti-rabbit IgG (Thermo Fisher), cells were resuspended in 200 l PBS/1% BSA and measured on a BD FACS Canto II flow cytometer (BD Bioscience)

pmid:22155089