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Description

In phase 2, acetaldehyde is metabolised by ALDH to the inert metabolite acetic acid

Pelleted cells were then resuspended in K-36 buffer (0.05 M KH 2 PO 4 , 0.05 M K 2 HPO 4 , 100 mM KCl, 15 mM NaCl, pH 7) and dounced (60 strokes) on ice