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T cells were first seeded onto a glass-bottom dish and brought into focus prior to APC addition

The balance between reduced glutathione (active form) and oxidised glutathione reflects overall redox status and the bodys ability to manage oxidative load

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Next, 900 L of serum extract was dried down in a vacuum evaporator (Thermo), reconstituted in 90 L of 50% acetonitrile (A955, Fisher Scientific) and transferred to glass vials for LC/MS analysis