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On analyzing the interaction of the co-crystallized ligand (Flavin-Adenine Dinucleotide, FAD) with the protein, we identified that FAD forms hydrogen bonding with Glu50, Ala130, Ser51, Thr57, Lys66, Thr339, Asp331, Gly31 and Ser30

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Collectively, these data demonstrate that adipocyte Ssu72 deficiency mitigates HFD-induced adipose tissue expansion, inflammation, and fibrosis in WAT

The primary hepatocytes were initially cultured in DMEM medium for 24 hours, after which the wells were washed once with PBS