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Description

Further, Huggins et al

Briefly, protein was extracted from isolated oocytes via direct incubation in SDS extraction buffer comprising 2% SDS (w/v), 10% sucrose (w/v) in 0.1875 M Tris, pH 6.8 and supplemented with ProteCEASE protease inhibitors (Cat #786326, G-Biosciences, MO, USA) and boiling (100 C for 5 min)