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Transwell cell migration and invasion assays The cells were resuspended in serum-free medium to a concentration of 2.5 10 5 cells/mL (huh7) or 4 10 5 cells/mL (Hep3B)

For endothelium, it has multiple functions, including, but not limited to, hemostasis, fibrinolysis, regulation of vascular tone, inflammatory cascades, and mediation of osmolality 29,30

This mechanism differs significantly from minoxidil, which simply dilates existing blood vessels

Its compact structure provides a focused means of investigating cell migration and tissue stabilization without broader transcriptional modulation

The intensity of the light was adjusted so that baseline TF latencies were approximately 3 s