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Administration of a GSH inhibitor into nucleus accumbens impairs PR test performance To assess whether decreasing local accumbal GSH levels alters motivated performance, rats were injected with either BSO (an inhibitor of gamma-glutamylcysteine synthetase-the rate-limiting enzyme for GSH synthesis) at 7 g/l or DMSO (the diluent of BSO) intra-NuAc (1 l per hemisphere) (Figure 4a and 4b)

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Available from: www.pnas.org/doi/10.1073/pnas.0402680101

Behavioral testing (open field, social interaction, tail flick nociception) was conducted between postnatal days 3040, followed by biochemical analyses in cerebral cortex, hippocampus, striatum, and cerebellum

1 2 "Oral and Intravenous Acetylcysteine for Treatment of Acetaminophen Toxicity: A Systematic Review and Meta-analysis"