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KC, Flam E, Cesare J, Cappola TP, Margulies K, Prosser B, Arany Z (2021) Truncated titin proteins in dilated cardiomyopathy

2.10 Overexpression and knockdown of RRM2 gene in TPC-1 and IHH4 cells For upregulating RRM2 expression in cells, we obtained a full-length RRM2 sequence fragment using a PCR method and subsequentlycloned it into vector LV18, verified by DNA sequencing and transfected 293T cells (see Supplementary Materials for details)