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Materials and methods Lung tissue specimens Lung tissue specimens from 72 patients (16 life-long non-smokers, 26 current smokers with mild-to-moderate COPD, 22 current smokers with normal lung function) operated on for local lung tumour (malignant and non-malignant such as hamartomas) and 8 ex-smokers with very severe (Stage IV) COPD undergoing lung transplantation formed the basis for immunohistochemical studies from Oulu and Helsinki University Hospitals