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This batch is verified at 99.643% purity by third-party analytical testing (HPLC for purity, mass spectrometry for identity)

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1 H NMR (300 MHz, CDCl 3 ): 2.32-2.50 (m, 1H), 2.85-2.30 (m, 1H), 3.87-3.95 (m, 1H), 4.27-4.32 (m, 1H), 4.54-4.67 (m, 2H), 5.29 (br s, 1H, disappeared in D20 exchange), 6.19 (d, J=2.1 Hz, 1H), 6.89 (dd, J=9.0 Hz, J=2.1 Hz, 1H), 7.35 (d, J=5.7 Hz, 1H), 7.83 (d, J=9.0 Hz, 1H), 8.19 (d, J=6.3 Hz, 1H)

This is compared to NMDA EPSCs that experienced no significant changes in amplitude when IGF-1 DES was administered [2]

Trimethylsilyl cyanide (52.0 mL, 580.0 mmol) was added dropwise to the stirred solution of #A2 (65.0 g, 290.0 mmol) and DBU (50.0 mL, 348.0 mmol) in THF (500 mL) at room temperature over a period of 15 minutes