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20 mL of this culture was supplemented with 1 mM NH 2 -Pap (from a 50 mM stock solution in 100 mM HCl) and, after 1 h, expression of the sfGFP gene (under control of the tet p/o ) was induced by adding 200 ng/mL anhydrotetracycline (aTc), followed by incubation at 30 C for 16 h

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Despite the discoveries identifying the indisputable role of the post-translational phosphorylation of tight junction proteins, future studies to decipher the kinases and phosphatases involved, in addition to the putative binding partners and proteinprotein interactions, are imperative for a better understanding of their regulatory mechanisms