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In addition, to confirm that REW did not induce formation of higher order assemblies followed by complement activation in the absence of cognate antigen, the antibodies were incubated in NHS at 37 C at a concentration of 100 g/mL before formation of IgG complexes and C4d was measured in ELISA (Fig

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The dosage chart in milliliters offers another perspective for researchers who prefer volumetric measurement over unit-based calculations

These allow to rapidly evaluate or in some cases even synthesise and evaluate protein variants as highlighted in AN385: Cell-free extract protein synthesis: Rapid single vessel DNA assembly and protein production in microliter scale

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