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To demonstrate that these effects were due to a reduced ROS content in NACET-pretreated cells, we performed a CellROX Green dye staining and Fluorescence-activated Cell Sorting (FACS) analysis immediately after H 2 O 2 or t-BOOH treatments and observed a reduction in the amount of ROS generated inside the cells by oxidative stress (Figure 5b,c,e,f)

Since the involvement of lactate transporters in the neuroprotective effect mediated by stimulation has been observed not only in our in vitro model but also in an in vivo rat model 36 and we have found that lactate itself has a neuroprotective effect in ischemic stroke, it might be a valid candidate approach for neuroprotection which exerts its effect on multiple therapeutic targets and can be easily administrated to patients

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