CloneTracker™ 5-10 Single Barcode Cell Labeling Kit - 5-10 Unique Constructs + PCR Primers and Reagents CRISPR Cells 000 protein coding genes

000 protein coding genes

Description Quantity Provided RSCOV-SPACK SARS-CoV-S Pseudotyped Lentiviral Packaging Mix 250 ug RSCOV2-SPACK SARS-CoV2-S Pseudotyped Lentiviral Packaging Mix 250 ug RSCOV2-SDGPACK SARS-CoV2-S-D614G Pseudotyped Lentiviral Packaging Mix 250 ug RSCOV2-SDGB117PACK SARS-CoV2-S-B

Alignment Software Supporting Files Library Configuration Files (JSON format

sgRNA targeting the copGFP fluorescent protein in a standard (no Cas9) lentiviral vector

The design is the same as our dual-sgRNA libraries in the pRSL10-mU6-sgNT1-hU6-sgNT2-UbiC-TagRFP vector

CloneTracker™ 5-10 Single Barcode Cell Labeling Kit - 5-10 Unique Constructs + PCR Primers and Reagents CRISPR Cells 000 protein coding genesChoose 5 or 10 lentiviral constructs with barcodes flanked by common primer sequences to facilitate PCR amplifications. After transduction and antibiotic selection of cells, barcodes can be amplified and sequenced to estimate the number of cells present from a specifically labeled population. In this way, multiple cell populations can be labeled, mixed, growth together, and then the representative numbers of descendant cells from each population can