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Kuhla et al., 2007)

2.2 Image processing and data analysis methods 2.2.1 Neutrophil extravasation, migration, and viability analysis The images from extravasation experiments were converted to max intensity projections in the Z-axis

Free Radic Biol Med 127:108115 Hussain SP, Aguilar F, Amstad P et al (1994) Oxy-radical induced mutagenesis of hotspot codons 248 and 249 of the human p53 gene

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To evaluate the effects of compound on GPX4 activity, compound was first pre-incubated with purified GPX4 in the assay buffer [Tris-HCl (100 mM, pH 7.4), EDTA (5 mM), Triton X-100 (0.1% v/v), NADPH (0.2 mM), glutathione (3 mM), and glutathione reductase (1 unit)] for 5 min at 37C