Dulbecco’s MEM (DMEM) Ham’s F-12 FBS PROTOCOLS FOR THAWING THE CELLS

PROTOCOLS FOR THAWING THE CELLS AND SUBCULTURE A) Pre-coating of T25 flasks- Add 2ml each Universal Coating Solution  (UBP-01) into a T25 flask to cover the whole surface of the flask

Gently rock the culture vessel to distribute the cells evenly

Minimal effect on product performance

Remove excess coating solution from the flask

Version: 2

Dulbecco’s MEM (DMEM) Ham’s F-12 FBS PROTOCOLS FOR THAWING THE CELLSDescription Product Name: Dulbeccos MEM (DMEM) Hams F 12 Catalog No: PG025 Product Format: Liquid Stored at 4C _____________________________________________________________________ General Information: Dulbeccos MEM is the most widely used modification of BME. It contains a 4 fold higher concentration of amino acids and vitamins. Non essential amino acids and certain essential trace elements were added. The bicarbonate concentration was increased. The