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10.1038/s41418-023-01125-0 154 YadavA

Abbreviations ATF4: Activating transcription factor 4 m: Mitochondrial membrane potential DCFH-DA: 2,7-dichlorofluorescin diacetate ECL: Enhanced chemiluminescence ELISA: Enzyme-linked immunosorbent assay ESI: Electrospray ionization FC: Fold change Fe: Ferrous iron Fer-1: Ferrostatin-1 GBM: Glioblastoma multiforme GSH: Glutathione HMDB: Human metabolome database HRP: Horseradish peroxidase IC50: Half-maximal inhibitory concentration KEGG: Kyoto Encyclopedia of Genes and Genomes K: Potassium ion LC-MS/MS: Liquid chromatographytandem mass spectrometry LDH: Lactate dehydrogenase Lip-1: Liproxstatin-1 MDA: Malondialdehyde Nrf2: Nuclear factor erythroid 2related factor 2 OH: Hydroxyl radicals PBS: Phosphate-buffered saline PCA: Principal component analysis PLS-DA: Partial least-squares discriminant analysis PVDF: Polyvinylidene difluoride QTOF: Quadrupole time-of-flight mass spectrometer Rb: Rubidium ion RbCl: Rubidium chloride RCD: Regulated cell death RIPA: Radioimmunoprecipitation assay ROS: Reactive oxygen species RT: Retention time SDS-PAGE: Sodium dodecyl sulfatepolyacrylamide gel electrophoresis TCA: Tricarboxylic acid TEM: Transmission electron microscopy TMZ: Temozolomide UPLC: Ultra-performance liquid chromatography References Louis DN, Perry A, Wesseling P, Brat DJ, Cree IA, Figarella-Branger D, et al

Moreover, the pCTV vector showed similar expression levels, when compared to pCDNA (Fig

For GUV experiments, raw rim binding of each domain was normalized by the fluorescence signal of a 1 M protein solution from the same purification batch to reduce variabilities in fluorescence from different purification batches